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An SDS-PAGE gel was run after each purification step

Importantly, pharmacokinetic analyses have demonstrated no significant differences in tirzepatide metabolism among healthy individuals, patients with renal impairment, 186 or those with hepatic dysfunction, 187 obviating the need for dose adjustments in these populations

Consistent with the growth inhibition, cell death significantly increased in BMS-754807 + U0126 treated cells than that in vehicle, BMS-754807, or U0126 treated cells (Fig