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Use follow-ups to fine-tune and stay consistent

Waste Disposal: Safe disposal of biological waste and sharp instruments requires dedicated sharps containers and biohazard disposal services, adding a small but necessary cost to the research protocol

Embrace darkness: Use blackout curtains or an eye mask to block out light

Interestingly, even heterozygous Bbs5 +/ mice, which displayed normal body composition and energy metabolism compared with WT controls (Supplemental Figure 1, KV), were resistant to leptin (Supplemental Figure 3, K, L, O, and P), suggesting that leptin resistance may serve as a potential biomarker even in individuals with a single Bbs gene mutation