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Two examples of organic DOX-loaded PLGA confirmed the nanoparticles anti-tumor efficacy: (i) a pilot-scale manufacturing process yielded strong anti-tumor efficacy in in vivo orthotopic model, with negligible blood toxicity at therapeutic concentrations (27) and (ii) a detailed confocal characterization of intracellular trafficking of DOX-loaded PLGA NPs in human U87MG describing NPs internalization by clathrin-mediated endocytosis (23)